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t25 tissue culture flasks (Sarstedt)

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Sarstedt t25 tissue culture flasks
T25 Tissue Culture Flasks, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t25+culture+flask/flasks+t75/pm42030331-51-7-11
Average 86 stars, based on 1 article reviews

t25 tissue culture flasks - by Bioz Stars, 2026-10

86/100 stars

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Saline:

Article Title: Three‐dimensional, PEG‐based hydrogels induce spheroid formation and enhance viability of A2058 melanoma cells
Article Snippet: Human microvascular endothelial cell line, HMEC‐1 (Invitrogen Life Technologies, Carlsbad, CA, USA), was cultured in MCDB‐131 medium, supplemented with 10% FBS (Biosera Europe, Nuaillé, France), 2% l ‐glutamine, 1% penicillin/streptomycin (Gibco, Invitrogen Corporation, New York, USA), 1 μg·mL −1 hydrocortisone, and 10 ng·mL −1 EGF (epidermal growth factor). .. The T25 culture flask (Sarstedt AG & Co. KG, Nümbrecht, Germany) was coated previously with 0.02% gelatin solved in PBS (phosphate buffer saline, pH 7.2). ..

Article Title: Three-dimensional, PEG-based hydrogels induce spheroid formation and enhance viability of A2058 melanoma cells.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 1% penicillin/streptomycin (Gibco, Invitrogen Corporation, New York, USA), 1 lg mL 1 hydrocortisone, and 10 ng mL 1 EGF (epidermal growth factor). .. The T25 culture flask (Sarstedt AG & Co. KG, N€umbrecht, Germany) was coated previously with 0.02% gelatin solved in PBS (phosphate buffer saline, pH 7.2). ..

Stable Transfection:

Article Title: A high-affinity split-HaloTag for live-cell protein labeling
Article Snippet: .. For the stable cell lines generated using Flp-IN TM T-REx TM system (Thermo Fisher Scientific), cells were grown in a T25 culture flask (Sarstedt AG & Co.KG) until ~80% confluency. .. Lipofectamine 3000 transfection, as described above, was performed by co-transfecting the pCDNA5/FRT plasmid encoding POI (440 ng) and the pOG44 plasmid encoding the Flp-recombinase (3560 ng, Invitrogen).

Generated:

Modification:

Article Title: Embroidered Silk Fibroin Scaffolds for ACL Tissue Engineering
Article Snippet: .. After explanting the LCLs, they were sliced into 2 mm 2 pieces and placed in a T25 culture flask (Sarstedt AG & Co. KG, Nürnbrecht, Germany) with growth medium (Dulbecco’s Modified Eagle’s Medium (DMEM)/Ham’s F12 medium (Pan-Biotech GmbH) supplemented with 10% fetal bovine serum (FBS, Pan-Biotech GmbH), 1% penicillin/streptomycin solution (Pan-Biotech GmbH), 25 μg/mL ascorbic acid (Sigma-Aldrich, Munich, Germany), 2.5 μg/mL amphotericin B (Pan-Biotech GmbH), and 1% MEM amino acid solution (Pan-Biotech GmbH) for several weeks. ..

Article Title: Embroidered Silk Fibroin Scaffolds for ACL Tissue Engineering
Article Snippet: .. After explanting the LCLs, they were sliced into 2 mm2 pieces and placed in a T25 culture flask (Sarstedt AG & Co. KG, Nürnbrecht, Germany) with growth medium (Dulbecco’s Modified Eagle’s Medium (DMEM)/Ham’s F12 medium (Pan-Biotech GmbH) supplemented with 10% fetal bovine serum (FBS, Pan-Biotech GmbH), 1% penicillin/streptomycin solution (Pan-Biotech GmbH), 25 μg/mL ascorbic acid (Sigma-Aldrich, Munich, Germany), 2.5 μg/mL amphotericin B (Pan-Biotech GmbH), and 1% MEM amino acid solution (Pan-Biotech GmbH) for several weeks. ..